TCMK-1 华体会体育电子购买

简要描述：北纳生物经营范围：AG华体会体育、国内外标准品、对照品、实验耗材、试剂、菌株、细胞、ATCC、培养基、血清、冶金标样、标准气体、试剂盒等，欢迎查询！ATCCCCL-139 TCMK-1 华体会体育电子购买

产品型号：ATCCCCL-139
厂商性质：生产厂家
更新时间：2019-04-17
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　　hthcom华体会是一家专业从事标准物质、计量分析仪器和精细化工产品研发及销售的科研单位。是国内标准物质提供商之一，代理销售国内外数万种标准物质、微生物、菌种、细胞类等产品。

　　北京标准物质网隶属于hthcom华体会，具备强大的分析测试、技术开发、*、物流管理和售后服务能力，并与国内外众多检测机构、科研院校和工厂企业有着良好和稳定的合作关系，具有较强的性和影响力。

北纳生物也隶属于hthcom华体会，实验基地总占地面积16亩，并设有细胞库、微生物菌种保藏库，细胞培养、菌种培养等实验室。拥有细胞和菌株2万余株，并设有生物资源信息库，收录信息达20余万株。致力于提供实验室综合解决方案，为广大客户服务。

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AG华体会体育、国内外标准品、对照品、实验耗材、试剂、菌株、细胞、ATCC、培养基、血清、冶金标样、标准气体、试剂盒等，欢迎查询！

ATCCCCL-139TCMK-1 华体会体育电子购买

基本信息
资源编号	ATCCCCL-139
资源名称	TCMK-1
种属	Mus musculus, mousekidney
类型	SV40 transformed
形态	epithelial
提供形式	frozen
致病类型	normal
安全等级	2

培养方法
培养基	The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
传代方法	Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subction Ratio: A subction ratio of 1:2 to 1:8 is recommended Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
生长条件	Temperature: 37°C
生长特性	adherent
存储条件	liquid nitrogen vapor phase;Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase

详细说明
描述	About 2% of the cells are positive for SV40 viral antigen, but virus is not recoverable. Tested and found negative for ectromelia virus (mousepox).
Age	weanling
Strain	C3H/Mai
Karyotype	modal number = 78; range = 64 to 157.All cells showed 2-6 acrocentric chromosomes with secondary constrictions and 1-4 biarmed chromosomes per cells. Sixty percent of the cells had one or more minute chromosomes.
Derivation	The cells are SV40 transformed and are positive for SV40 T-antigen.
Antigen Expression	H-2k
Tumorigenic	No
Effects	No, in immunosuppressed mice Yes, in semisolid medium
Virus Susceptibility	Vesicular stomatitis, Glasgow (Indiana) Human poliovirus 1
Virus Resistance	poliovirus 1
Year of Origin	1963

ATCCCCL-139TCMK-1 华体会体育电子购买

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